From www.cyclingnews.com:
"Next, Mr. Suh turned to Ms. Frelat's stage 17 B sample analysis:
"Next, Mr. Suh turned to Ms. Frelat's stage 17 B sample analysis:
Suh: You said you were involved in the testing of the blank and the actual sample?
Frelat: Yes.
Suh: When you did the first analysis, did you use the OS2 automatic background subtraction feature?
Frelat: When the report was drawn up I would always verify the estimate of the background noise and the integration of the peaks?
Suh: And you would do that manually?
Frelat: Yes.
Suh: You are choosing where the peaks begin and where they end?
Frelat: Yes.
Suh: Your SOP says you have exercise care in picking where the peaks begin and end because it can have a dramatic impact on the isotopic value of the results?
Frelat: Yes, it is written that we must be very careful but not that it will have dramatic consequences or that it will affect the results in a dramatic way. It is written that it could have a significant change in the results.
Suh: What do you believe is a significant difference in carbon isotope testing for your final per mil value?
Frelat: A difference of 1.5 or 1.6 per mil.
Suh: You are aware that LNDD would report an adverse analytic finding (AAF) whenever there is a greater than negative 3 per mil difference?
Frelat: Yes.
Suh: So 1.5 or 1.6 is half of that? Can you point to a SOP where it defines a significant difference as 1.5 of 1.6?
Frelat: It is not written, my answer was concerning my opinion.
Suh: Of course, you were trying to do your very best job?
Frelat: Yes.
Suh: You are saying that the ones that are close, are close enough?
Frelat: Yes.
Suh: Are you saying those pages document your choices of where the peaks are for the stage 17 analysis?
Frelat: This is the report of the results which I have found.
Suh: Is there a report of how you came to that conclusion of where the peaks begin and where they end?
Frelat: We do not print any reports that show the 44/45 trace. We only have the final report after we have done the work and the chromatogram with the highest ion.
Suh: For stage 17, did you use manual subtraction?
Frelat: Yes, I just said that."
So if I understand Frelat's testimony correctly, there is no permanent record of how the integration was performed to arrive at the final 13C/12C ratio???
Last edited by:
Andrew Coggan: May 18, 07 14:10